B1758
Biocytin hydrochloride
≥98% (TLC), suitable for drug transporter assays
Synonym(s):
Nε-Biotinyl-L-lysine
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About This Item
Empirical Formula (Hill Notation):
C16H28N4O4S · xHCl
CAS Number:
Molecular Weight:
372.48 (free base basis)
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.26
MDL number:
Product Name
Biocytin hydrochloride, ≥98% (TLC)
Quality Level
assay
≥98% (TLC)
form
powder
technique(s)
drug transporter assay: suitable
color
white to off-white
storage temp.
−20°C
SMILES string
Cl.NC(CCCCNC(=O)CCCCC1SCC2NC(=O)NC12)C(O)=O
InChI
1S/C16H28N4O4S.ClH/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14;/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24);1H
InChI key
FMGQMNFQNPUYBS-UHFFFAOYSA-N
Application
- Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog.: This paper highlights the application of biocytin hydrochloride in tracing retinal projections in amphibians. The study demonstrates the efficacy of biocytin hydrochloride in neuroanatomical mapping, facilitating research in visual system development and function (Scalia et al., 1997).
Biochem/physiol Actions
Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Margaret A MacNeil et al.
The Journal of comparative neurology, 506(1), 6-15 (2007-11-09)
The biocytin wide-field bipolar cell in rabbit retina has a broad axonal arbor in layer 5 of the inner plexiform layer and a wide dendritic arbor that does not contact all cones in its dendritic field. The purpose of our
Barry Wolf et al.
Molecular genetics and metabolism, 86(1-2), 44-50 (2005-09-10)
Biotinidases from various species ranging from fungi and insects to human have specific amino acids, and regions that are evolutionarily conserved. These specific amino acids and regions are further supported by their homology to a variety of amidases and nitrilases
Alessandro R Galloni et al.
eLife, 9 (2020-05-29)
Anatomical similarity across the neocortex has led to the common assumption that the circuitry is modular and performs stereotyped computations. Layer 5 pyramidal neurons (L5PNs) in particular are thought to be central to cortical computation because of their extensive arborisation