H2519
Heparinase I from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin lyase I, Heparinase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein (enzyme + BSA)
conjugate
conjugate (Glucosaminoglycan)
Quality Level
form
lyophilized solid
specific activity
≥200 units/mg protein (enzyme + BSA)
mol wt
42.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
Heparinase I from Flavobacterium heparinum has been used:
- in the digestion of plasma samples
- to study the effect of medium, washing and sonication on the infectivity of Chlamydia pneumonia
- in enzymatic removal of heparan sulfate from the surface of human rhabdomyosarcoma cells
Biochem/physiol Actions
Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.
Other Notes
One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Cardiopulmonary Bypass: Principles and Practice, 509-509 (2008)
Methodological aspects affecting the infectivity of Chlamydia pneumoniae in cell cultures in vitro
Yan Y, et al.
Journal of Microbiological Methods, 61(1), 127-130 (2005)
HeparinHeparin - A Century of Progress, 273-273 (2012)