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P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

Name: Phospholipase C, Phosphatidylinositol-specific from <I>Bacillus cereus</I>
Brand: SIGMA

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

Synonym(s):

1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC

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About This Item

CAS Number:

37288-19-0

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-638-2

MDL number:

MFCD00131896

EC Number:

4.6.1.13 (BRENDA, IUBMB)

Specific activity:

≥1,000 units/mg protein (Lowry)

Biological source:

Bacillus sp. (Bacillus cereus)

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Properties

biological source

Bacillus sp. (Bacillus cereus)

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

≥1,000 units/mg protein (Lowry)

mol wt

28 kDa

foreign activity

Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein

storage temp.

2-8°C

Gene Information

Bacillus cereus E33L ... BCZK3513(3026815)

Description

General description

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Application

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
to cleave immunolabeled HeLa cells

Biochem/physiol Actions

Used for the release of GPI anchored proteins from the membrane.

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Physical form

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Analysis Note

Acetylcholinesterase is measured according to Ellman, et al.

Other Notes

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000469923

0000427386

0000119053

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