1.00888
Clostridium perfringens selective supplement
For the preparation of TSC Agar, For the enumeration of Clostridium perfringens
Synonym(s):
Clostridium perfringens Supplement
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106212
Quality Segment
agency
AOAC® (976.30:2023), APHA, EN, FDA-BAM (Medium M169), GB (4789.13:2012), ISO (14189:2013), ISO (15213-2:2023), ISO (7937:2004 (withdrawn)), ISO (TS 15213-3:2024)
form
lyophilized powder
technique(s)
microbiological culture: suitable
solubility
83 g/L
application(s)
food and beverages
microbiology
compatibility
for use with TSC agar (Base) (1.11972)
storage temp.
2-8°C
suitability
Clostridium spp.
General description
Clostridium perfringens selective supplement contains the antibiotic D-Cycloserine that inhibits the accompanying bacterial flora and causes the developing colonies to remain smaller. It also reduces the diffusion and the disturbing blackening around the Clostridium perfringens colonies. 4-Methylumbelliferylphosphate (MUP) is a fluorogenic substrate for the alkaline and acid phosphatase. The acid phosphatase is a high specific indicator for Clostridium perfringens, and it splits the fluorogenic substrate MUP. The fluorescence of resulting product, 4-methylumbelliferone, can be identified in long wave UV-light, which suggests the presence of Clostridium perfringens.
Application
Clostridium perfringens selective supplement is used for the enrichment of TSC-Agar (Base) (1.11972) during the enumeration of Clostridium perfringens in food products and other materials.
Other Notes
Replaces: 1008880017
Legal Information
AOAC is a registered trademark of AOAC International
Storage Class
11 - Combustible Solids
WGK
WGK 3
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