A8275
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Goat Anti-Rabbit IgG (whole molecule)–HRP
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
77
biological source
goat
conjugate
peroxidase conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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General description
IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.
Immunogen
Purified rabbit IgG.
Application
Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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G A De Ruiter et al.
Journal of general microbiology, 139(7), 1557-1564 (1993-07-01)
A monoclonal antibody (mAb) was raised against extracellular polysaccharides from Mucor racemosus after intrasplenic immunization of mice. An indirect ELISA and a dot-blot assay were developed with this mAb. The IgG antibody was found to be very specific for all
Yu-Xin Bai et al.
Medical science monitor : international medical journal of experimental and clinical research, 23, 5774-5782 (2017-12-06)
BACKGROUND Lung alveolar epithelial type II cells (AEC II) are the most important stem cells in lung tissues, which are critical for wound repair of bronchopulmonary dysplasia (BPD). This study investigated the effects of calcitonin gene-related peptide (CGRP) on AEC
L Micheli et al.
Analytical and bioanalytical chemistry, 373(8), 678-684 (2002-08-24)
In this paper the production of antibodies against saxitoxin (STX) is described, as is the optimization and comparison of two competitive ELISA formats (direct and indirect) for the detection of this toxin. Tests were performed in a 96-well microplate using
Karina Bravo-Tello et al.
PloS one, 12(11), e0187696-e0187696 (2017-11-09)
Soybean meal has been used in many commercial diets for farm fish; despite this component inducing intestinal inflammation. On the other hand, microalgae have increasingly been used as dietary supplements in fish feed. Nevertheless, the vast quantity of microalgae species
Immunoblotting and immunodetection.
Gallagher S, Winston SE, Fuller SA
Current Protocols in Immunology, doi: 10-doi: 10 (2001)
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