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A9655

Auramine O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
2465-27-2
Molecular Weight:
303.83
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24891484
EC Number:
219-567-2
MDL number:
MFCD00012484
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061
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Product Name

Auramine O, Dye content ≥80 %, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O, 432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

wgk

WGK 3

Storage Class

11 - Combustible Solids

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT6123
SHBT0566
SHBS7131
SHBR0463
SHBQ6204

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E W Koneman.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)
E Kohen.
Cell Structure and Function by Microspectrofluorometry, 490-490 (2014)
Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).
Inbaraj B S, et al.
Biochemical Engineering Journal, 31(3), 204-215 (2006)
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