B2025
Brilliant Blue G - Colloidal Concentrate
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About This Item
NACRES:
NA.85
UNSPSC Code:
12352200
Related Categories
General description
Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.
Application
Brilliant Blue G - Colloidal Concentrate has been used in the staining
- protein fragments post in vitro pepsin digestion
- ovarian tumor fluid proteome prior to profiling
- the transcription factor (TFIID) complex from Saccharomyces cerevisiae
Preparation Note
The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.
Analysis Note
This product has been tested for suitability on SDS-PAGE.
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
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Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis
Candiano G, et al.
Electrophoresis, 25(9), 1327-1333 (2004)
Proteomic analysis of ovarian cancer tumor fluid is a rich source of potential biomarkers
Poersch A, et al.
Journal of Proteomics and Bioinformatics, 5(2), 2-2 (2014)
A proteomic signature of ovarian cancer tumor fluid identified by highthroughput and verified by targeted proteomics
Poersch A, et al.
Journal of proteomics, 145(2), 226-236 (2016)
Elena Barbieri et al.
Journal of aging research, 2011, 845379-845379 (2011-06-02)
This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional and biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mitochondrial mass and membrane potential were
Staining ability and biocompatibility of brilliant blue G: preclinical study of brilliant blue G as an adjunct for capsular staining
Hisatomi T, et al.
Archives of Ophthalmology, 124(4), 514-519 (2006)
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