H3375
HEPES
≥99.5% (titration)
Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
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About This Item
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
230-907-9
Beilstein/REAXYS Number:
883043
MDL number:
Quality Level
assay
≥99.5% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
color
white
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 500 mg/mL, clear, colorless
suitability
suitable for component for culture media, suitable for enzyme extraction
application(s)
advanced drug delivery
diagnostic assay manufacturing
general analytical
life science and biopharma
pharmaceutical
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
General description
HEPES, also known as 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer. It is effective in the pH 6.8-8.2 range, with a pKa of 7.48 at 25°C. Recognized as one of the best all-purpose buffers, especially in cell biology, biochemical, and biological research, HEPES is a dipolar ionic buffer and one of the Good′s buffers that does not form significant complexes with most metal ions. This property makes it suitable as a non-coordinating buffer in solutions with metal ions.
Widely utilized in cell culture, HEPES excels at maintaining physiological pH despite fluctuations in carbon dioxide concentration produced by cellular respiration, a feature not as prominent in bicarbonate buffers. It serves as an ampholytic separator for creating pH gradients in isoelectric focusing and exhibits less interference with DNA-restriction enzyme reactions compared to buffers with fewer substituted amine groups, such as Tris. Beyond these applications, HEPES may find application in various biological and biochemical studies, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility makes it an indispensable tool in diverse research applications.
Widely utilized in cell culture, HEPES excels at maintaining physiological pH despite fluctuations in carbon dioxide concentration produced by cellular respiration, a feature not as prominent in bicarbonate buffers. It serves as an ampholytic separator for creating pH gradients in isoelectric focusing and exhibits less interference with DNA-restriction enzyme reactions compared to buffers with fewer substituted amine groups, such as Tris. Beyond these applications, HEPES may find application in various biological and biochemical studies, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility makes it an indispensable tool in diverse research applications.
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Application
HEPES has been used:
- As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
- As a component of Hanks′ solution used in the degradation testing of Iron
- As a component of HEPES medium in the transfection of cell culture with plasmids
- Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
- As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies
Features and Benefits
- High purity product for biochemical and biological research
- Suitable for Cell culture
- Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
Other Notes
Go to Redi-Dri™ Product Listing RDD002
Easily compare specifications for HEPES products with the HEPES specification table.
Easily compare specifications for HEPES products with the HEPES specification table.
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Legal Information
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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