S0692
Spectinomycin
Ready Made Solution, 100 mg/mL in DMSO/H2O, 1:1
Synonym(s):
Actinospectacin
biological source
Streptomyces sp.
form
ready-to-use solution
concentration
100 mg/mL in DMSO/H2O, 1:1
color
colorless
solubility
DMSO/H2O, 1:1: soluble 100 mg/mL
antibiotic activity spectrum
Gram-negative bacteria, Gram-positive bacteria, mycobacteria
mode of action
protein synthesis | interferes
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Application
Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.
Biochem/physiol Actions
Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).
Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.
Disclaimer
Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.
General description
Chemical structure: aminoglycoside
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas
Preparation Note
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.
Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
201.0 °F - closed cup
flash_point_c
93.9 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Ausubel, F.M., et al.
Current Protocols in Molecular Biology, 1-1 (1987)
A C Chang et al.
Journal of bacteriology, 134(3), 1141-1156 (1978-06-01)
Construction and characterization of a class of multicopy plasmid cloning vehicles containing the replication system of miniplasmid P15A are described. The constructed plasmids have cleavage sites within antibiotic resistance genes for a variety of commonly employed site-specific endonucleases, permitting convenient
New tools for chloroplast genetic engineering.
H Daniell
Nature biotechnology, 17(9), 855-856 (1999-09-03)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
Holloway, W.J.
Med. Clin. North Amer., 66, 169-173 (1982)
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