B4501

Biotin

Name: Biotin
Brand: SIAL

≥99% (HPLC), lyophilized powder

Synonym(s):

D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₆N₂O₃S

CAS Number:

58-85-5

Molecular Weight:

244.31

NACRES:

NA.21

PubChem Substance ID:

24891778

eCl@ss:

34058011

UNSPSC Code:

12352201

EC Number:

200-399-3

MDL number:

MFCD00005541

Beilstein/REAXYS Number:

86838

Product Name

Biotin, ≥99% (HPLC), lyophilized powder

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

biological source

synthetic (organic)

assay

≥99% (HPLC)

form

lyophilized powder

technique(s)

HPLC: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

ammonium hydroxide: 50 mg/mL (2 M)

storage temp.

2-8°C

Quality Level

300

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Related Categories

Biochemicals

Cell Culture Supplements & Reagents

Coenzymes

Proteins & Enzymes

Vitamins

Application

Biotin may be used to elute proteins from avidin/streptavidin resins. Biotin has been used:

for culturing of oligodendrocytes.
as a vitamin supplement for the growth of Bacillus species.
for blocking endogenous biotin during immunohistology procedures.

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are is required for various applications. Biotin also works as a cofactor/coenzyme of carboxylating enzymes, such as pyruvate carboxylase, acetyl-CoA carboxylases 1 and 2, 3-methylcrotonyl-CoA carboxylase (MCC), and propionyl-CoA carboxylase (PCC). It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A, etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry, and flow cytometry. This approach is also applied for to the purification and characterization of various molecules of interest. Biotin is involved in the epigenetic regulation of genes, cell signaling, and the structure of chromatin.

General description

Biotin is a water-soluble vitamin. Mammals depend on biotin via plant and microbial dietary sources. Biotin consists of two rings that are attached to valeric acid moiety as a side chain. One of the rings is attached to ureido group and the other ring is the tetrahydrothiophene group.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000518465

0000516128

0000511410

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Find documentation for the products that you have recently purchased in the Document Library.

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The biotin-(strept)avidin system: principles and applications in biotechnology.

Diamandis EP and Christopoulos TK

Clinical Chemistry, 37, 625-625 (1991)

Biotin: biochemical, physiological and clinical aspects

Said H M

Sub-Cellular Biochemistry, 56, 1-19 (2012)

Isolation and culture of rat and mouse oligodendrocyte precursor cells.

Chen Y et al.

Nature Protocols, 2, 1044-1044 (2007)

Exercise induces interleukin-8 receptor (CXCR2) expression in human skeletal muscle.

Lone Frydelund-Larsen et al.

Experimental physiology, 92(1), 233-240 (2006-10-13)

Exercise induces a marked increase in interleukin-8 (IL-8) mRNA and protein expression within skeletal muscle fibres. Interleukin-8 belongs to a subfamily of CXC chemokines containing a Glu-Leu-Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo

Development of peptide-based lineage-specific serology for chronic Chagas disease: geographical and clinical distribution of epitope recognition.

Tapan Bhattacharyya et al.

PLoS neglected tropical diseases, 8(5), e2892-e2892 (2014-05-24)

Chagas disease, caused by infection with the protozoan Trypanosoma cruzi, remains a serious public health issue in Latin America. Genetically diverse, the species is sub-divided into six lineages, known as TcI-TcVI, which have disparate geographical and ecological distributions. TcII, TcV

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