C6423
α-Chymotrysin Protease
Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010112
NACRES:
NA.26
EC Number:
232-671-2
MDL number:
Product Name
α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder
grade
Proteomics Grade
form
salt-free, lyophilized powder
mol wt
25 kDa
suitability
suitable for protein sequencing
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
cow ... CTRB1(618826)
Related Categories
General description
α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.
Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.
Application
α-Chymotrypsin from bovine pancreas is used for the following applications:
- Protein Identification by mass spectrometry (MS)
- The isolation and characterization of myosin heavy chains
- Toxin preparation
- The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE
Biochem/physiol Actions
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
α-Chymotrypsin from bovine pancreas is stabilized by Ca2+ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu2+ and Hg.
Other Notes
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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The α-chymotrypsin-catalyzed Povarov reaction: one-pot synthesis of tetrahydroquinoline derivatives.
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Methods in molecular biology. v. 16
Burrell, MM
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Johnston et al.
The Journal of experimental biology, 201 (Pt 12), 623-646 (1998-02-06)
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