E4643
Heparin-Binding EGF-Like Growth Factor human
≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
Synonym(s):
hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor
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About This Item
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Assay:
≥97% (SDS-PAGE and N-terminal analysis)
Form:
lyophilized powder
Mol wt:
9.5 kDa
Product Name
Heparin-Binding EGF-Like Growth Factor human, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
≤3 ng/mL
quality
endotoxin tested
mol wt
9.5 kDa
packaging
pkg of 50 and 250 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
Biochem/physiol Actions
Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
F Vinante et al.
Blood, 94(9), 3169-3177 (1999-11-11)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a widely expressed EGF superfamily member that induces mitogenic and/or chemotactic activities toward different cell types through binding to EGF receptors 1 or 4. Membrane-bound HB-EGF exerts growth activity and adhesion capabilities
M Lanzrein et al.
The Biochemical journal, 310 ( Pt 1), 285-289 (1995-08-15)
Preincubation of Vero cells with 1 microM phorbol 12-myristate 13-acetate (PMA) decreased the specific binding of diphtheria toxin by about 50%, whereas the toxic effect, endocytic uptake and membrane translocation were completely blocked. Toxin bound to PMA-treated cells was released
R Iwamoto et al.
The Journal of biological chemistry, 274(36), 25906-25912 (1999-08-28)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) transduces mitogenic signals through the EGF receptor (EGFR). There are two forms of HB-EGF, the membrane-anchored form (pro-HB-EGF) and the soluble form (sHB-EGF). We studied the biological activity of pro-HB-EGF by using a