I2911
IMDM
StableCell™, with stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Iscove’s Modified Dulbecco’s Medium, IMDM
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About This Item
Product Name
StableCell™ IMDM, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
impurities
Endotoxin, tested
components
NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.
Application
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Legal Information
StableCell is a trademark of Sigma-Aldrich Co. LLC
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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