T3405
3,3′,5,5′-Tetramethylbenzidine dihydrochloride
chromogenic, tablet
Synonym(s):
4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
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About This Item
Empirical Formula (Hill Notation):
C16H20N2 · 2HCl
CAS Number:
Molecular Weight:
313.27
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
264-769-6
MDL number:
Product Name
3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet
InChI
1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChI key
NYNRGZULARUZCC-UHFFFAOYSA-N
SMILES string
Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
form
tablet
mp
≥300 °C
storage temp.
2-8°C
Quality Level
Related Categories
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
- anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
- anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
- in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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CD45 isoform expression is associated with different susceptibilities of human naive and effector CD4+ T cells to respond to IL-4
Stutz A, et al.
European Journal of Immunology, 35(2), 575-583 (2005)
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C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production, simple engineering, and the possibility to obtain higher
Shurong Tang et al.
Mikrochimica acta, 185(5), 273-273 (2018-05-01)
A hybrid material composed of graphitic carbon nitride (g-C3N4) and platinum nanoparticles (PtNPs) with peroxidase mimicking activity was used to design a rapid, sensitive and low-cost colorimetric method for the determination of Ag(I). The g-C3N4-PtNPs hybrid was synthesized by reduction
A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients
Alamdari DH, et al.
Clinical Biochemistry, 40(3-4), 248-254 (2007)
Beth C Holbrook et al.
Virology, 476, 124-133 (2014-12-30)
Respiratory infection of young infants results in increased morbidity and mortality compared to infection of adults. In spite of the significance of this health issue, our understanding of the immune response elicited in infants especially in the respiratory tract is
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