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UFC901024 Amicon Ultra-15, membrana PLGC Ultracel-PL, 10 kDa

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Replacement Information

Tabla espec. clave

Key ApplicationsLímite de peso molecular nominal (NMWL)VolumenVolumen de concentrado final mínimo
Extracción de proteínas, Purificación de proteínas10 KDa15 mL200 µL
Número de catálogoUFC901024
Nombre comercial
  • Amicon Ultra
DescripciónAmicon Ultra-15, membrana PLGC Ultracel-PL, 10 kDa
Información preliminarAMICON ULTRA-15 15ML - 10 KDa cutoff. Available in 3 pack sizes.
UFC901008 - 8 pack
UFC901024 - 24 pack
UFC901096 - 96 pack

High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
High retentate recovery of greater than 90%
Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
Heat-sealed membrane minimizes downstream extractables
100% integrity tested for reliable performance
Convenient sample monitoring with translucent housing and volume gradations
Direct pipettor sample access eliminates processing step to recover concentrate
High concentration factors of 80–100X

Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
Concentration of dilute or pre-purified proteins from column eluents
Desalting, buffer exchange and protein dialysis
Referencias bibliográficas
Información del producto
Código HS 8421 29 90
Código del filtroPLGC
Código de coloresTransparente con tapa rosa
Tipo de filtroUltrafiltración
Límite de peso molecular nominal (NMWL)10 KDa
Quality LevelMQ400
Aplicación Ultracel-10 regenerated cellulose membrane, 15 mL sample volume
Aplicaciones clave
  • Extracción de proteínas
  • Purificación de proteínas
Notas de aplicaciónRotor de cabezal basculante: 4.000 x g; Rotor de ángulo fijo (35°): 5.000 g
Información biológica
EsterilidadNo estéril
Información fisicoquímica
Aplicación centrífugaProtein Concentration and Desalting
Longitud12.1 cm (4.807 in.)
Diámetro2.97 cm
Área de filtración7.6 cm²
Volumen de concentrado final mínimo200 µL
Volumen15 mL
Información de materiales
  • Regenerated Cellulose (RC)
Material del dispositivo
  • Housing: Styrene/butadiene
  • Tubo: Polipropileno
  • Tapa: Polietileno
Material de soporteCelulosa regenerada
Información toxicológica
Información de seguridad según el GHS
Información de seguridad
Declaraciones de uso del producto
Información de almacenamiento y transporte
Información sobre embalaje
Información de transporte
Información complementaria
Global Trade Item Number
Número de referencia GTIN
UFC901024 04054839076633


Referencias bibliográficas

Visión general referenciasPub Med ID
Large-Scale Filter-Aided Sample Preparation Method for the Analysis of the Ubiquitinome.
Casanovas A, Pinto-Llorente R, Carrascal M, Abian J
Anal Chem  89(7)  3840-3846  2016

Mostrar resumen
28260372 28260372
A complex of Protocadherin-19 and N-cadherin mediates a novel mechanism of cell adhesion.
Emond, Michelle R, et al.
J. Cell Biol., 195: 1115-21 (2011)  2010

Mostrar resumen
22184198 22184198

Certificados de calidad

UFC901024 0000234282


REFINE PROTEIN PREPARATION. Tools for better protein analysis
Western Blotting Tools


A centrifugal ultrafiltration-based method for enrichment of microvesicles

Preguntas frecuentes

Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the maximum sample volume for Amicon Ultra?
Amicon Ultra-4Amicon Ultra-15
Swinging Bucket4ml 15ml
Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
3.5ml(23 degree rotor angle)
What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15?
Swinging Bucket4000 x g4000 x g
3500 x g for concentrated/viscous solutions
Fixed Angle(23, 35 &45 degrees)7500 x g5000 x g
What is the chemical compatibility of Amicon Ultra?Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support.
Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.

Manuales del usuario

Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide

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Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units