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48-684MAG MILLIPLEX MAP Ras-Raf Oncoprotein Magnetic Bead Panel 6-Plex - Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

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Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-684MAG
96-well plate  96-well plate
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Descripción

Replacement Information

Tabla espec. clave

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
BRAF (Ser446) CRAF (Ser338) MEK1 (Ser217/Ser221) Ras (total) RasG12D RasG12V HMplexLuminex xMAP
Description
Catalogue Number48-684MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Ras-Raf Oncoprotein Magnetic Bead Panel 6-Plex - Cell Signaling Multiplex Assay
OverviewRas proteins are GTPases encoded by three genes: HRAS, KRAS, and NRAS. The protein products of these genes exhibit a high degree of sequence identity and are regulated by a conserved mechanism: exchange of GDP for GTP switches the Ras protein into an active conformation. In its GTP-bound state, Ras has high affinity for its downstream effectors, including the Raf family of protein kinases (ARAF, BRAF, and CRAF/RAF1). Raf proteins, in turn, signal through MEK to activate the MAPK pathway. In healthy cells, Ras activation is a transient response to extracellular growth stimuli and is restricted by the rapid conversion of Ras-GTP back to Ras-GDP. However, the Ras-Raf-MEK pathway is often aberrantly activated in cancer. Mutations in the RAS genes are amongst the most frequently encountered oncogenic events, occurring in ~25% of human cancers, with mutations altering the 12th amino acid (glycine) being particularly prevalent. These driver mutations maintain Ras in a constitutively active, GTP-bound state. Additionally, certain cancers feature KRAS gene amplification, although the effects of gene amplification on protein expression are not yet well-characterized.

The MILLIPLEX® MAP 6-plex Ras-Raf Oncoprotein Magnetic Bead Kit (Cat. No. 48-684MAG) is a premixed kit to be used for the simultaneous quantification of the following 6 protein analytes:

• pBRAF (Ser446)
• pCRAF (Ser338)
• pMEK1 (Ser217/Ser221)
• Ras (total)
• Mutant Ras protein, RasG12D*
• Mutant Ras protein, RasG12V*
*Detects H-Ras, N-Ras, and K-Ras protein
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-assay:< 15
  • Inter-assay: < 20
Panel TypeCell Signaling
Applications
ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enable the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This kit must be run using Assay Buffer 2 (provided).
• This assay requires 25 µL diluted cell lysate per well.
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • BRAF (Ser446)
  • CRAF (Ser338)
  • MEK1 (Ser217/Ser221)
  • Ras (total)
  • RasG12D
  • RasG12V
Physicochemical Information
Standard Curve Range
  • 0.13–2,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
48-684MAG 04061841049098