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03-0207-00 SMC® Human PD-1 High Sensitivity Immunoassay Kit

The SMC® Human PD-1 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Programmed Cell-Death Protein 1 (PD-1) in Human Serum and K2EDTA Plasma samples.

03-0207-00
96 well assay  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Catalogue Number03-0207-00
Trade Name
  • SMC®
DescriptionSMC® Human PD-1 High Sensitivity Immunoassay Kit
OverviewThe SMC® Human PD-1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Background Information
The SMC® Human PD-1 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Programmed Cell-Death Protein 1 (PD-1) in Human Serum and Plasma samples. A capture antibody specific for Human PD-1 has been pre-coated onto paramagnetic microparticles (beads). The user pipettes beads, standards, and samples into uncoated microplate wells. During incubation, the PD-1 present in the sample binds to the capture antibody on the coated beads. Unbound molecules are washed away during the subsequent wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to PD-1 that has been captured onto the beads, thus completing the immunosandwich. Elution buffer is then added and incubated. The elution buffer dissociates the bound protein sandwich from the beads surface releasing the labeled antibodies. These antibodies are separated during transfer to a 384-well read plate. The plate is loaded into the SMCxPRO® System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of PD-1 present in the sample when captured. The amount of PD-1 in unknown samples is interpolated from a standard curve.
References
Product Information
Components
  • Assay Buffer
    PD-1 Antibody Coated Beads
    Standard Diluent
    PD-1 Detection Antibody
    PD-1 Standard
    10X Wash Buffer
    Buffer D
    Elution Buffer B
Detection method
Quality LevelMQ200
Applications
ApplicationThe SMC® Human PD-1 High Sensitivity Immunoassay Kit utilizes Single Molecule Counting technology, based on the trusted sandwich immunoassay principle, to digitally measure Programmed Cell-Death Protein 1 (PD-1) in Human Serum and K2EDTA Plasma samples.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesProgrammed cell death protein 1 (PD-1), CD279, is a type I membrane protein found in T cells, B cells, and macrophages that binds Programmed cell death ligand 1 and 2 (PD-L1 and PD-L2). PD-1 is found in both soluble and insoluble forms. PD-1 regulates the body’s immune response through a variety of pathways, down-regulating immune system activity and suppressing T-cell inflammatory activity. PD-1 has been implicated as playing a role in cancers, autoimmune diseases, the immune response to viruses, and in immunological pathways related to brain repair. As an immune checkpoint, PD-1 promotes apoptosis of antigen-specific T-cells in lymph nodes and reduces apoptosis in regulatory T-cells. Blockers of the PD-1 pathway have been approved as cancer therapeutics. We are proud to offer the new SMC® Human PD-1 High Sensitivity Immunoassay Kit for use with the state-of-the-art SMCxPRO® detection system. Researchers can accurately assess PD-1 concentrations even in low abundance in human serum and plasma samples, enabling studies that examine the relevance of this key analyte from health to disease.
This new streamline kit has the following features:
• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Rigorously developed to achieve accurate and reproducible performance on the state-of-the-art SMCxPRO® ultrasensitive immunoassay system
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
03-0207-00 0

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