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208880 Anti-Calnexin, C-Terminal (575-593) Rabbit pAb

208880
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Gp, Ht, H, Mk, M, Rb, R, ShRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
208880-50UL Plastic ampoule 50 ul
Description
OverviewRecognizes the ~90 kDa calnexin protein.
Catalogue Number208880
Brand Family Calbiochem®
SynonymsAnti-IP90, Anti-p88
Application Data
Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
References
ReferencesHammond, C., et al. 1994. J. Cell Biol. 126, 41.
Ou, W.-J., et al. 1993. Nature 364, 771.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Positive controlHeat-shocked HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
ImmunogenCanine
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Guinea Pig
  • Hamster
  • Human
  • Monkey
  • Mouse
  • Rabbit
  • Rat
  • Sheep
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Calnexin, C-Terminal (575-593) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Calnexin, C-Terminal (575-593) Rabbit pAb Certificates of Analysis

TitleLot Number
208880

References

Reference overview
Hammond, C., et al. 1994. J. Cell Biol. 126, 41.
Ou, W.-J., et al. 1993. Nature 364, 771.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision31-January-2008 JSW
SynonymsAnti-IP90, Anti-p88
ApplicationImmunoblotting (1 µg/ml)
Application Data
Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~90 kDa calnexin protein.
BackgroundCalnexin is an ER transmembrane protein that transiently associates with newly synthesized, incompletely folded monomeric glycoproteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRµ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
HostRabbit
Immunogen speciesCanine
Immunogena synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
IsotypeIgG
Speciesbovine, canine, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep
Positive controlHeat-shocked HeLa cells
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHammond, C., et al. 1994. J. Cell Biol. 126, 41.
Ou, W.-J., et al. 1993. Nature 364, 771.
David, V., et al. 1993. J. Biol. Chem. 268, 9585.
Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.