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217692 CDC25 Phosphatase Inhibitor II, NSC 663284 - CAS 383907-43-5 - Calbiochem

217692
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Overview

Replacement Information

Key Spec Table

Empirical FormulaCAS #
C₁₅H₁₆ClN₃O₃383907-43-5

Products

Catalogue NumberPackaging Qty/Pack
217692-5MG Plastic ampoule 5 mg
Description
OverviewA cell-permeable 7-substituted quinolinedione compound that displays antiproliferative properties. Acts as a potent, irreversible and mixed competitive inhibitor of the CDC25 phosphatase family (Ki = 29 nM, 95 nM and 89 nM for CDC25A, CDC25B2 and CDC25C, respectively; IC50 = 210 nM for CDC25B2) and displays nearly 20- and 450-fold greater selectivity for the CDC252 phosphatases over VHR and PTP1B (IC50 = 4.0 µM and > 100 µM, respectively). Reported to arrest cell cycle progression, inhibit Cdk dephosphorylation and delay tumor growth (IC50 = ~1.5 µM in NCI-60 tumor cell panel). Shown to modify CDC25A catalytic domain and to block cellular ERK dephosphorylation in HeLa-CDC25A cells.
Catalogue Number217692
Brand Family Calbiochem®
Synonyms6-Chloro-7-(2-morpholin-4-yl-ethylamino)quinoline-5,8-dione, DA3003-1, PTP Inhibitor XXI, VHR Inhibitor II
References
ReferencesChao, J. I., et al. 2004. J. Biol. Chem. 279, 20267.
Han, Y., et al. 2004. J. Pharmacol. Exp. Ther. 309, 64.
Pu, L., et al. 2002. J. Biol. Chem. 277, 46877.
Lazo, J.S., et al. 2001. J. Med. Chem. 44, 4042.
Product Information
CAS number383907-43-5
ATP CompetitiveN
FormRed solid
Hill FormulaC₁₅H₁₆ClN₃O₃
Chemical formulaC₁₅H₁₆ClN₃O₃
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetCD25A
Primary Target K<sub>i</sub>29 nM, 95 nM and 89 nM for CDC25A, CDC25B2 and CDC25C, respectively; 210 nM for CDC25B2
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Carcinogenic / Teratogenic
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Ethanol stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

CDC25 Phosphatase Inhibitor II, NSC 663284 - CAS 383907-43-5 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

CDC25 Phosphatase Inhibitor II, NSC 663284 - CAS 383907-43-5 - Calbiochem Certificates of Analysis

TitleLot Number
217692

References

Reference overview
Chao, J. I., et al. 2004. J. Biol. Chem. 279, 20267.
Han, Y., et al. 2004. J. Pharmacol. Exp. Ther. 309, 64.
Pu, L., et al. 2002. J. Biol. Chem. 277, 46877.
Lazo, J.S., et al. 2001. J. Med. Chem. 44, 4042.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-November-2010 JSW
Synonyms6-Chloro-7-(2-morpholin-4-yl-ethylamino)quinoline-5,8-dione, DA3003-1, PTP Inhibitor XXI, VHR Inhibitor II
DescriptionA cell-permable, potent, irreversible, and competitive inhibitor of the Cdc25 phosphatase family (Ki = 29 nM, 95 nM, and 89 nM for Cdc25A, Cdc25B2, and Cdc25C, respectively) with anti-proliferative properties. Displays nearly 20- and 450-fold greater selectivity over VHR and PTP1B (IC50 = 4.0 µM and > 100 µM, respectively). Reported to arrest cell cycle progression, inhibit Cdk dephosphorylation, and delay tumor growth (mean IC50 = 1.5 µM in NCI-60 tumor cell panel). Also shown to block ERK dephosphorylation caused by ectopic Cdc25A expression.
FormRed solid
Intert gas (Yes/No) Packaged under inert gas
CAS number383907-43-5
Chemical formulaC₁₅H₁₆ClN₃O₃
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (5 mg/ml) or Ethanol (5 mg/ml). Use only fresh, anhydrous DMSO.
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month at -20°C. Ethanol stock solutions are stable for up to 6 months at -20°C.
Toxicity Carcinogenic / Teratogenic
ReferencesChao, J. I., et al. 2004. J. Biol. Chem. 279, 20267.
Han, Y., et al. 2004. J. Pharmacol. Exp. Ther. 309, 64.
Pu, L., et al. 2002. J. Biol. Chem. 277, 46877.
Lazo, J.S., et al. 2001. J. Med. Chem. 44, 4042.