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420220 iPSC Induction Enhancer, Thiazovivin - Calbiochem

420220
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Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₅H₁₃N₅OS

Products

Catalogue NumberPackaging Qty/Pack
420220-10MG Plastic ampoule 10 mg
Description
OverviewA cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) as a ROCK inhibitor (71% inhibition at 2 µM). Effectively prevents trypsinization-induced apoptosis in hESC cultures by stabilizing newly synthesized cell surface E-cadherin and cell-cell adhesion. Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated hESCs even in the absence of ECM. Greatly augments SB431542 (Cat. No. 616461) and PD0325901 (Cat. No. 444966) in improving iPSCs generation efficiency from 4-TF- (Oct44, Klf4, Sox2, and c-Myc) transfected fibroblast cultures.
Catalogue Number420220
Brand Family Calbiochem®
SynonymsN-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
References
ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
Lin, T., et al. 2009. Nat. Methods 6, 805.
Product Information
FormTan powder
Hill FormulaC₁₅H₁₃N₅OS
Chemical formulaC₁₅H₁₃N₅OS
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationiPSC Induction Enhancer, Thiazovivin, CAS 1226056-71-8, is a cell-permeable ROCK inhibitor that prevents trypsinization-induced apoptosis in hESC cultures.
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

iPSC Induction Enhancer, Thiazovivin - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

iPSC Induction Enhancer, Thiazovivin - Calbiochem Certificates of Analysis

TitleLot Number
420220

References

Reference overview
Xu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
Lin, T., et al. 2009. Nat. Methods 6, 805.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-April-2012 JSW
SynonymsN-Benzyl-2-(pyrimidin-4-ylamino)thiazole-4-carboxamide, Induced Pluripotent Stem Cells Induction Enhancer, Rho Kinase Inhibitor XII
DescriptionA cell-permeable aminothiazolo-carboxamide compound that is ~5-fold more potent than Y-27632 (Cat. Nos. 688000 & 688001) in inhibiting ROCK enzymatic activity (71% inhibition at 2 µM). Single-cell dissociation of cultured hESCs is demonstrated to hyperactivate Rho-ROCK signaling and result in cell death, while suppression of such hyperactivation by Tzv (2 µM) or Y-27632 (2 µM) stabilize newly synthesized cell surface E-cadherin, which in turn promotes cell-cell adhesion and prevents trypsinization-induced apoptosis (12% vs 81% TUNEL-positive cells after trypsinization in matrigel cultures with 2 µM Tzv or DMSO, respectively). Unlike, Ptn (Cat. No. 544049), Tzv protects dissociated individual hESCs even in the absence of ECM (24% vs 90% TUNEL-positive cells after trypsinization in non-coating plates with 2 µM Tzv or Ptn, respectively). Simultaneous blockage of TGFβ and MEK-ERK pathways by 2 µM SB431542 (Cat. No. 616461) and 0.5 µM PD0325901 (Cat. No. 444966) is reported to result in >50-fold improvement of human iPSCs (induced Pluripotent Stem Cells) generation from fibroblast cultures virally transfected with 4-TF (Oct44, Klf4, Sox2, and c-Myc), while the inclusion of 0.5 µM Tzv on top of SB431542 and PD0325901 is demonstrated to further improve the efficiency to an overall improvement of >200-fold from 4-TF-induction alone.
FormTan powder
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₅H₁₃N₅OS
Structure formulaStructure formula
Purity≥98% by HPLC
SolubilityDMSO (100 mg/ml)
Storage +2°C to +8°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesXu, Y., et al. 2010. Proc. Natl. Acad. Sci. USA 107, 8129
Lin, T., et al. 2009. Nat. Methods 6, 805.