341738
Sulforhodamine B, acid form
Dye content 95 %
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About This Item
Empirical Formula (Hill Notation):
C27H30N2O7S2
CAS Number:
Molecular Weight:
558.67
NACRES:
NA.23
PubChem Substance ID:
UNSPSC Code:
12352103
EC Number:
220-025-2
MDL number:
form
solid
Quality Level
composition
Dye content, 95%
mp
>300 °C (lit.)
λmax
558 nm
SMILES string
CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1
InChI
1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)
InChI key
IOOMXAQUNPWDLL-UHFFFAOYSA-N
General description
Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself into the solution under mild acidic conditions.
Application
pH sensitive of SRB towards it reactivity to living cells renders it useful for scaling drug induced cytotoxicity and cell proliferations. SRB assay was used to monitor the enzymatic activity of living cells.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Lindsay N Sanford et al.
Analytical biochemistry, 434(1), 26-33 (2012-11-13)
Accurate control of the sample temperature during thermal cycling is critical for successful polymerase chain reaction (PCR). Direct sensor contact with the reaction is problematic, forcing measurements external to the sample and compromising accuracy during rapid temperature transitions. The widespread