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Merck

05-1059

Sigma-Aldrich

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

culture supernatant, clone 8.78.8, from rabbit

Synonym(s):

CSAID-binding protein, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase MXI2, MAP kinase p38 alpha, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, cytokine suppressive anti-inflammato

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

8.78.8, monoclonal

species reactivity

human, vertebrates, mouse

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_001306

UniProt accession no.

Q15759

shipped in

wet ice

target post-translational modification

phosphorylation (pThr180/pTyr182)

Gene Information

human ... MAPK14(1432)

General description

38 kDa
The p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Immunogen

Epitope: Phosphorylated Thr180/Tyr182
Synthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.

Application

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.
Research Sub Category
MAP Kinases

Inflammation & Autoimmune Mechanisms

Biochem/physiol Actions

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
This antibody detects p38α when phosphorylated on Thr180/Tyr182.

Physical form

Rabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.

Analysis Note

Evaluated by western blot on anisomycin-treated HeLa cell lysate.
Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tamer S Kaoud et al.
Nature communications, 10(1), 5232-5232 (2019-11-21)
Recently, the targeting of ERK with ATP-competitive inhibitors has emerged as a potential clinical strategy to overcome acquired resistance to BRAF and MEK inhibitor combination therapies. In this study, we investigate an alternative strategy of targeting the D-recruitment site (DRS)
Yu-Ling Tai et al.
PloS one, 11(9), e0163617-e0163617 (2016-09-23)
Growth factor receptor bound protein-7 (Grb7) is a multi-domain adaptor protein that is co-opted by numerous tyrosine kinases involved in various cellular signaling and functions. The molecular mechanisms underlying the regulation of Grb7 remain unclear. Here, we revealed a novel
Thuy Nguyen Thi et al.
International journal of oncology, 65(1) (2024-06-07)
CD46, a transmembrane protein known for protecting cells from complement‑mediated damage, is frequently dysregulated in various types of cancer. Its overexpression in bladder cancers safeguards the cancer cells against both complement and antibody‑mediated cytotoxicity. The present study explored a new
M W Lopes et al.
Translational psychiatry, 6(12), e986-e986 (2016-12-14)
Glucocorticoids (GC) released during stress response exert feedforward effects in the whole brain, but particularly in the limbic circuits that modulates cognition, emotion and behavior. GC are the most commonly prescribed anti-inflammatory and immunosuppressant medication worldwide and pharmacological GC treatment

Global Trade Item Number

SKUGTIN
05-105904053252327872

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