AP136P
Goat Anti-Rat IgG Antibody, HRP conjugate
Chemicon®, from goat
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA, IHC, WB
Species reactivity:
rat
Citations:
75
Technique(s):
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Related Categories
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Routinely evaluated on Enzyme-linked Immunosorbent Assay.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used..
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used..
Application
Detect Rat IgG using this Goat anti-Rat IgG Antibody, HRP conjugate validated for use in ELISA, IH & WB.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Specific for rat IgG, heavy and light chain.
General description
Goat anti-rat IgG affinity purified antibody, HRP conjugated.
Physical form
Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA.
Reconstitute with 2 mL of sterile distilled water.
Reconstitute with 2 mL of sterile distilled water.
Preparation Note
Maintain lyophilized product at 2-8°C for up to 12 months.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mark A Csandl et al.
Drug metabolism and disposition: the biological fate of chemicals, 44(6), 857-866 (2016-04-14)
Active efflux of both drugs and organic anion metabolites is mediated by the multidrug resistance proteins (MRPs). MRP1 (ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and MRP4 (ABCC4) have partially overlapping substrate specificities and all transport 17β-estradiol 17-(β-d-glucuronide) (E217βG). The cysteinyl leukotriene
Felix Wiedmann et al.
Molecular biology of the cell, 30(12), 1425-1436 (2019-04-11)
Two pore-domain potassium (K2P) channels mediate potassium background currents that stabilize the resting membrane potential and facilitate action potential repolarization. In the human heart, hK2P17.1 channels are predominantly expressed in the atria and Purkinje cells. Reduced atrial hK2P17.1 protein levels
Counteraction of tetherin antiviral activity by two closely related SIVs differing by the presence of a Vpu gene.
Nikovics, K; Dazza, MC; Ekwalanga, M; Mammano, F; Clavel, F; Saragosti, S
Testing null
N V Andreeva et al.
Cell stress & chaperones, 21(4), 727-733 (2016-04-20)
Heat shock proteins including the major stress protein HSP70 support intracellular homeostasis and prevent protein damage after a temperature increase and other stressful environmental stimuli, as well as during aging. We have shown earlier that prolonged administration of recombinant human
Quan Chen et al.
Molecular medicine reports, 14(1), 202-208 (2016-05-14)
Ultraviolet (UV) radiation-induced immunosuppression leading to skin cancer has received increased attention in previous years. The present study aimed to investigate the immunoprotection offered by Anthelios sunscreen in a mouse model of Candida albicans‑induced delayed‑type hypersensitivity. Anthelios sunscreen was applied
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AP136P | 04053252511882 |
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