31149
Deoxyribonucleic acid, low molecular weight from salmon sperm
Synonym(s):
Salmon sperm dsDNA, low molecular weight DNA, DNA
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About This Item
CAS Number:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
309-566-6
NACRES:
NA.52
MDL number:
Biological source:
fish
Form:
lyophilized powder
Solubility:
50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
Storage temp.:
2-8°C
biological source
fish
grade
Molecular Biology, puriss. p.a.
form
lyophilized powder
impurities
≤5% protein
solubility
50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
absorbance ratio
A260/280 nm ~1.4
storage temp.
2-8°C
Application
Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
- to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.
- in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.
- as a precursor in the synthesis of the bio-dots.
Biochem/physiol Actions
Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Andrea Hájková et al.
Bioelectrochemistry (Amsterdam, Netherlands), 116, 1-9 (2017-03-18)
A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual
J J Gao et al.
Journal of immunology (Baltimore, Md. : 1950), 166(11), 6855-6860 (2001-05-22)
LPS is well recognized for its potent capacity to activate mouse macrophages to produce TNF-alpha, an important inflammatory mediator in bacterial infection-related diseases such as septic shock. We demonstrate here that while inducing only low levels of TNF-alpha alone, DNA
Thiol modification of silicon-substituted hydroxyapatite nanocrystals facilitates fluorescent labelling and visualisation of cellular internalisation
Williams RL et al
Journal of Material Chemistry B: Materials for Biology and Medicine, 35, 4370-4378 (2013)
Eiji Sunami et al.
Clinical chemistry, 55(3), 559-567 (2009-01-10)
Prostate cancer (PCa) detection using serum-based prostate specific antigen (PSA) is limited by frequent false-positive and -negative results. Genetic aberrations such as allelic imbalance (AI) and epigenetic changes such as promoter hypermethylation have been detected in circulating DNA of cancer
V N Kondratova et al.
Biochemistry. Biokhimiia, 74(11), 1285-1288 (2009-11-18)
A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters
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