A9543
Bovine Serum Albumin
heat shock fraction, low endotoxin, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, electrophoresis: suitable, western blot: suitable
Impurities:
≤1.0 EU/mg endotoxin
biological source
bovine
assay
≥98%
form
lyophilized powder
Quality Level
Gene Information
bovine ... ALB(280717)
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable, electrophoresis: suitable, western blot: suitable
impurities
≤1.0 EU/mg endotoxin
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
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Application
This A9543 BSA product has been used in various systems, including:
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
- NRK-52E rat kidney epithelioid cells: Ochiai, H. et al., J. Virol., 67(11), 6811-6814 (1993)
- Neonatal porcine pancreatic islets: Ellis, C.E. et al., Islets, 5(5), 216-225 (2013)
- Mouse:
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
- iPSC cell line: Martin, H.C. et al., Science, 362(6419), 1161-1164 (2018).
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Heat shock fractionated
- Low endotoxin
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Anna Barkaway et al.
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The migration of neutrophils from the blood circulation to sites of infection or injury is a key immune response and requires the breaching of endothelial cells (ECs) that line the inner aspect of blood vessels. Unregulated neutrophil transendothelial cell migration
Marcos V A S Navarro et al.
PLoS biology, 9(2), e1000588-e1000588 (2011-02-10)
The bacterial second messenger bis-(3'-5') cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which in Pseudomonas fluorescens requires the transmembrane receptor LapD. LapD exhibits a
Manoj K Pandey et al.
Molecular pharmacology, 75(3), 525-533 (2008-12-24)
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Tânia Veiga et al.
Eukaryotic cell, 11(2), 238-249 (2011-12-14)
The industrial production of penicillin G by Penicillium chrysogenum requires the supplementation of the growth medium with the side chain precursor phenylacetate. The growth of P. chrysogenum with phenylalanine as the sole nitrogen source resulted in the extracellular production of
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