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Merck

04-773

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

culture supernatant, clone SA19, from rabbit

Synonyme(s) :

Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Nom du produit

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal, culture supernatant, clone SA19, from rabbit

biological source

rabbit

antibody form

culture supernatant

antibody product type

primary antibodies

clone

SA19, monoclonal

species reactivity

rat, chicken, mouse, human

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_002471.1

UniProt accession no.

O14974

shipped in

dry ice

target post-translational modification

phosphorylation (pThr850)

Quality Level

100

Gene Information

human ... PPP1R12A(4659)

Analysis Note

Control
HEK293 cell lysate phosphorylated by ROKα/ROCKII.
Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).

Application

Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.

Biochem/physiol Actions

Predicted to cross-react with mouse and rat.
Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.

General description

Doublet at ~130 kDa
Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.

Immunogen

Epitope: pThr850(Ch) or pThr853(H)
KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 05-773

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Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Ebbe Boedtkjer et al.
Circulation, 124(17), 1819-1829 (2011-09-29)
Disturbances in pH affect artery function, but the mechanistic background remains controversial. We investigated whether Na(+), HCO₃- transporter NBCn1, by regulating intracellular pH(pH₁), influences artery function and blood pressure regulation. Knockout of NBCn1 in mice eliminated Na+, HCO₃⁻ cotransport and
E V Bouzinova et al.
Acta physiologica (Oxford, England), 224(1), e13059-e13059 (2018-02-27)
The Na,K-ATPase is involved in a large number of regulatory activities including cSrc-dependent signalling. Upon inhibition of the Na,K-ATPase with ouabain, cSrc activation is shown to occur in many cell types. This study tests the hypothesis that acute potentiation of
Huaqing Zheng et al.
Acta pharmaceutica Sinica. B, 11(12), 3994-4007 (2022-01-14)
Vascular smooth muscle cell (VSMC) migration plays a critical role in the pathogenesis of many cardiovascular diseases. We recently showed that TMEM16A is involved in hypertension-induced cerebrovascular remodeling. However, it is unclear whether this effect is related to the regulation
Vincent Sauzeau et al.
Methods in molecular biology (Clifton, N.J.), 1527, 213-218 (2017-01-25)
Increased arterial tone and the resulting rise in peripheral vascular resistance are major determinants of the elevated arterial pressure in hypertension. The RhoA/Rho kinase signaling pathways are now recognized as a major regulator of vascular smooth muscle contraction and arterial
Aditi U Gurkar et al.
Nature communications, 4, 2189-2189 (2013-07-24)
The Ser/Thr Rho kinase 1 (ROCK1) is known to have major roles in a wide range of cellular activities, including those involved in tumour metastasis and apoptosis. Here we identify an indispensable function of ROCK1 in metabolic stress-induced autophagy. Applying

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RéférenceGTIN
04-77304053252326356

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