07-641
Anti-MAP2K1/MEK1 Antibody
Upstate, rabbit polyclonal
Synonyme(s) :
Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1
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A propos de cet article
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
immunoprecipitation (IP)
western blot
western blot
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
Citations:
37
Uniprot accession no.:
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Laissez-nous vous aiderNom du produit
Anti-MEK1 Antibody, Upstate®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAP2K1(5604)
Preparation Note
2 years at -20°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.
Application
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.
Biochem/physiol Actions
MEK1
Predicted to cross-react with rabbit based on sequence homology.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
45kDa
Immunogen
Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.
Other Notes
Replaces: 04-376
Physical form
70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Brian J Girard et al.
The Journal of biological chemistry, 292(1), 339-350 (2016-11-25)
Cytoplasmic localization of proline, glutamic acid, leucine-rich protein 1 (PELP1) is observed in ∼40% of women with invasive breast cancer. In mouse models, PELP1 overexpression in the mammary gland leads to premalignant lesions and eventually mammary tumors. In preliminary clinical
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.
Teague, M A, et al.
Proceedings of the National Academy of Sciences of the USA, 83, 7371-7375 (1986)
cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.
Kosako, H, et al.
The Embo Journal, 12, 787-794 (1993)
Jason M Warfel et al.
Toxins, 3(10), 1278-1293 (2011-11-10)
Systemic anthrax disease is characterized by vascular leakage pathologies. We previously reported that anthrax lethal toxin (LT) induces human endothelial barrier dysfunction in a cell death-independent manner with actin stress fiber formation and disruption of adherens junctions (AJs). In the
Felice D'Agnillo et al.
PloS one, 8(4), e62576-e62576 (2013-04-30)
Vascular leakage pathologies such as pleural effusion and hemorrhage are hallmarks of anthrax pathogenesis. We previously reported that anthrax lethal toxin (LT), the major virulence factor of anthrax, reduces barrier function in cultured primary human microvascular endothelial cells. Here, we
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 07-641 | 04053252621413 |
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