217669
Anti-CD28 Mouse mAb (ANC28.1/5D10)
liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10
Synonyme(s) :
Anti-CD28 Antibody, CD28 Detection Antibody, Mouse Anti-CD28
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A propos de cet article
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
ANC28.1/5D10, monoclonal
Species reactivity:
human
Application:
—
Citations:
6
Service technique
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mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
ANC28.1/5D10, monoclonal
assay
>95% (immunoglobulin, SDS-PAGE)
form
liquid
contains
500 μg/mL gentamycin sulfate as preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
dilution
(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation
T cell Co-stimulation )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CD28(940)
General description
Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
Recognizes CD28 on human T cells and BHK cells transfected with human CD28.
Immunogen
Human
human CD28 Ig fusion protein and HPB-MLT human tumor cells
Application
Flow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
T cell Co-stimulation (see comments)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
T cell Co-stimulation (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
Preparation Note
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Other Notes
Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.
Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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