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Merck

217669

Anti-CD28 Mouse mAb (ANC28.1/5D10)

liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10

Synonyme(s) :

Anti-CD28 Antibody, CD28 Detection Antibody, Mouse Anti-CD28

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Nom du produit

Anti-CD28 Mouse mAb (ANC28.1/5D10), liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

ANC28.1/5D10, monoclonal

assay

>95% (immunoglobulin, SDS-PAGE)

form

liquid

contains

500 μg/mL gentamycin sulfate as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(Flow Cytometry (5 µg/mL)
Frozen Sections
Immunoprecipitation
T cell Co-stimulation )

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CD28(940)

Application

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.
Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
Recognizes CD28 on human T cells and BHK cells transfected with human CD28.

Immunogen

Human
human CD28 Ig fusion protein and HPB-MLT human tumor cells

Other Notes

Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.
Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Renée M Marshall et al.
Journal of immunology (Baltimore, Md. : 1950), 175(10), 6402-6411 (2005-11-08)
Stimulation of primary human T lymphocytes results in up-regulation of cyclin T1 expression, which correlates with phosphorylation of the C-terminal domain of RNA polymerase II (RNAP II). Up-regulation of cyclin T1 and concomitant stabilization of cyclin-dependent kinase 9 (CDK9) may
Mengting Chen et al.
International journal of biological sciences, 19(16), 5160-5173 (2023-11-06)
Rosacea is a common inflammatory skin disorder mediated by the dysregulation of both keratinocytes and T cells. Here, we report that aquaporin 3 (AQP3), a channel protein that mediates the transport of water/glycerol, was highly expressed in the epidermis and
Xiaofei Gao et al.
Frontiers in immunology, 13, 799331-799331 (2022-03-18)
Trace element iron affects T cell biology, but the knowledge about the role of iron in regulating Treg cell expansion is limited. Treg cells play an important role in keeping peripheral T cell tolerance, increasing Treg cell expansion is a
Zijun Wang et al.
The Journal of clinical investigation, 130(7), 3717-3733 (2020-03-20)
T follicular helper (Tfh) cells are indispensable for the formation of germinal center (GC) reactions, whereas T follicular regulatory (Tfr) cells inhibit Tfh-mediated GC responses. Aberrant activation of Tfh cells contributes substantially to the pathogenesis of autoimmune diseases, such as
Ruifang Wu et al.
The Journal of clinical investigation, 128(6), 2551-2568 (2018-05-15)
Immune imbalance of T lymphocyte subsets is a hallmark of psoriasis, but the molecular mechanisms underlying this aspect of psoriasis pathology are poorly understood. Here, we report that microRNA-210 (miR-210), a miR that is highly expressed in both psoriasis patients
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