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HEPES

Name: HEPES
Brand: MM

≥99% (alkalimetric), solid, pH 6.8-8.2, Molecular Biology

Synonyme(s) :

Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)

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A propos de cet article

Formule empirique (notation de Hill) :

C₈H₁₈N₂O₄S

Numéro CAS:

7365-45-9

Poids moléculaire :

238.30

NACRES:

NA.25

UNSPSC Code:

12161704

MDL number:

MFCD00006158

Beilstein/REAXYS Number:

883043

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Propriétés

Nom du produit

HEPES, A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

grade

Molecular Biology

Quality Level

100

description

Merck USA index - 14, 4654

assay

≥99% (alkalimetric)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 400 mg/mL, aqueous base: soluble

cation traces

heavy metals: ≤0.0005%

foreign activity

DNase, RNase, and protease, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

Application

Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides: This innovative study demonstrates the application of molecular biology grade HEPES in the formulation of bioactive probes for the fluorescent labeling of root cap cells. Using cellulose biosynthesis inhibition herbicides as a model system, this research provides valuable insights into plant biology, aiding in the understanding of plant cell wall structures and functions. The use of HEPES helps maintain the physiological pH during the experiments, ensuring the stability and effectiveness of the chemical probes used (Tamogami S et al., 2021).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Classe de stockage

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c