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Merck

539131

Protease Inhibitor Cocktail I

lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases

Synonyme(s) :

Protease Inhibitor Mix, Protease inhibitor

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A propos de cet article

UNSPSC Code:
12352200
NACRES:
NA.28
Form:
lyophilized
Solubility:
water: soluble
Storage temp.:
−20°C

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Nom du produit

Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

solubility

water: soluble

shipped in

wet ice

storage temp.

−20°C

Catégories apparentées

Application

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.
Protease Inhibitor Cocktail Set I have been used as a supplement in:
  • cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
  • RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
  • sucrose solution to homogenize mice cortices for myelin isolation

Biochem/physiol Actions

Inhibits a broad range of proteases and esterases.
Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Disclaimer

Toxicity: Harmful (C)

Features and Benefits

  • Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
  • Ready for reconstitution

General description

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

Other Notes

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Classe de stockage

8B - Non-combustible corrosive hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Ela B, et al.
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