ESG1107
Protéine LIF de souris recombinante ESGRO®
ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^7 units/ml.
Synonyme(s) :
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, Murine LIF, Mouse LIF, mLIF, mLIF
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A propos de cet article
UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32160801
Form:
liquid
Assay:
>95% (active component mLIF, SDS-PAGE)
Service technique
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assay
>95% (active component mLIF, SDS-PAGE)
form
liquid
manufacturer/tradename
Chemicon®
concentration
10000000 units/mL
technique(s)
cell culture | stem cell: suitable
input
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
Gene Information
mouse ... LIF(16878)
General description
Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
Application
SUGGESTED PROTOCOLS:
ES Cells: Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).
Physical form
Format : purifié
Preparation Note
®
A cold pack may be included for customer relations, however it is truly not needed. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. ° Freeze-thawing will reduce potency. ° Freeze-thawing should be avoided. °
Analysis Note
Activité spécifique: ®& μ
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay:
Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay:
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
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Classe de stockage
12 - Non Combustible Liquids
Certificats d'analyse (COA)
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