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Merck

71376

Chlorure de sodium

BioUltra, Molecular Biology, ≥99.5% (AT)

Synonyme(s) :

Halite, NaCl

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A propos de cet article

Formule linéaire :
NaCl
Numéro CAS:
7647-14-5
Poids moléculaire :
58.44
UNSPSC Code:
12352302
NACRES:
NA.25
PubChem Substance ID:
57652294
EC Number:
231-598-3
Beilstein/REAXYS Number:
3534976
MDL number:
MFCD00003477
Assay:
≥99.5% (AT)
Grade:
Molecular Biology
Form:
solid
Solubility:
H2O: 1 M at 20 °C, clear, colorless

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Nom du produit

Chlorure de sodium, BioUltra, Molecular Biology, ≥99.5% (AT)

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

InChI

1S/ClH.Na/h1H;/q;+1/p-1

SMILES string

[Na+].[Cl-]

grade

Molecular Biology

vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

product line

BioUltra

assay

≥99.5% (AT)

form

solid

availability

not available in (Sales restrictions may apply)

technique(s)

mass spectrometry (MS): suitable
western blot: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

801 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

2.16 g/cm3 at 25 °C (77 °F)

anion traces

bromide (Br-): ≤50 mg/kg
hexacyanoferrate(II) ([Fe(CN)6]4-): ≤1 mg/kg
iodide (I-): ≤10 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.01
λ: 280 nm Amax: ≤0.01

suitability

suitable for component for culture media
suitable for molecular biology

application(s)

genomic analysis

Quality Level

200

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Catégories apparentées

Application

Sodium chloride has been used:

  • as a component of buffer solution to prepare the pH solutions for open-circuit potential and cyclic voltammetry
  • in the preparation of Bold’s basal medium (BBM)
  • as a component of transcription and annealing buffer
  • to maintain osmotic balance and ensure the purity and stability of the isolated extracellular vesicles (EVs)
  • as a buffer for the lamination of lateral flow strips in the fabrication of lateral flow devices

Biochem/physiol Actions

Sodium chloride helps to stimulate the stable induction of T-helper cell 17 (TH17) cells.

Features and Benefits

  • Suitable for molecular biology and biochecmial research
  • Free from DNase, RNase, NICKase and protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
  • Tested for the presence of Anionic and Cationic traces

General description

Sodium chloride, an essential nutrient, plays a critical role in biochemistry and molecular biology, making significant contributions to various biochemical applications. Within biological systems, it serves as a fundamental electrolyte, regulating osmotic pressure, pH balance, and electrical conductivity, all of which are essential for proper cellular function and vital physiological processes. Furthermore, sodium chloride plays a pivotal part in maintaining protein structure and promoting enzymatic activity, essential for stabilizing protein conformations and catalyzing biochemical reactions. Its role extends to the regulation of cellular homeostasis and the facilitation of ion and nutrient transport across cell membranes.It is an integral component of buffers like PBS and SSC, where it aids in various biochemical procedures, including the creation of density gradients and the fine-tuning of biological functions. Notably, sodium chloride is used to increase the ionic strength in buffers and culture media, impacting the ionic environment crucial for specific experiments and cell culture. Moreover, it is applied in DNA precipitation from samples containing SDS and the removal of small nucleic acid fragments during plasmid DNA preparations. Its utility extends to cell culture applications, where it plays a role in bacterial, yeast, and cell culture growth media.In molecular biology and cell culture, sodium chloride is an indispensable salt that finds its place in various aspects of research. It is a common constituent in cell culture media, lysis buffers, and protein storage solutions, contributing to the maintenance of optimal conditions for cell growth and experimentation. The sodium chloride used in these applications is of high purity, rigorously tested for impurities such as DNases, RNases, phosphatases, proteases, and trace metals, making it the ideal choice for molecular biology and biochemical research

Other Notes

Component of lysis buffer and extraction buffer in the large-scale phenol extraction of RNA; Simple salting out procedure for extracting DNA from human nucleated cells
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Sales restrictions may apply
This product is intended for research purposes only, and it is not meant for human consumption.

Classe de stockage

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN7035
BCCN0724
BCCK6232
BCCH1572
BCCG0684

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria (Peltigerales, Ascomycota)
Cornejo C, et al.
Bio-protocol (2015)
A simple salting out procedure for extracting DNA from human nucleated cells.
S A Miller et al.
Nucleic acids research, 16(3), 1215-1215 (1988-02-11)
D.M. Wallace
Methods in Enzymology, 152, 39-39 (1987)
A solid-state thin-film Ag/AgCl reference electrode coated with graphene oxide and its use in a pH sensor
Kim T, et al.
Sensors, 15(3), 6469-6482 (2015)
Sodium chloride drives autoimmune disease by the induction of pathogenic T H 17 cells
Kleinewietfeld M, et al.
Nature, 496(7446), 518-518 (2013)
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Contenu apparenté

Sodium Chloride Specification Comparison

Sodium Chloride Specification Comparison

Data Sheet - 71376

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