D3571
Dipeptidyl Peptidase III human
recombinant, expressed in Sf9 cells
Synonyme(s) :
DPP III
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A propos de cet article
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥400 units/μg protein
Recombinant:
expressed in Sf9 cells
Concentration:
≥0.1 mg/mL
Service technique
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Laissez-nous vous aiderrecombinant
expressed in Sf9 cells
Quality Segment
form
solution
specific activity
≥400 units/μg protein
mol wt
82 kDa
concentration
≥0.1 mg/mL
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... DPP3(10072)
Application
Human dipeptidyl peptidase III has been used in a study to assess the effect of entropy-driven binding of opioid peptides on large domain motion in human dipeptidyl peptidase III. Human dipeptidyl peptidase III has also been used in a study to investigate Ets-1/Elk-1 as a critical mediator of its transcription in human glioblastoma cells.
Biochem/physiol Actions
DPP III is a cytosolic zinc-exopeptidase that is involved in the intracellular protein catabolism of eukaryotes. The enzyme is a monomeric acidic protein with a molecular mass of approximately 82,000 Da and a pI of 4.5-4.6. It is sensitive to freezing and temperatures above 40 °C. It is found to be inhibited by metallo-chelators and sulfydryl reagents. The activity can be restored by divalent cations and thiol compounds. It has a particularly high affinity for angiotensin III. It acts as a post-proline-cleaving enzyme on endomorphins.
Physical form
Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol and 3 mM DTT.
Other Notes
One unit will hydrolyze 1.0 picomole of Arg-Arg-AMC per minute at pH 7.5 at 25 deg °C
Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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