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Merck

G1544

Anti-GFP, N-terminal antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-Green Fluorescent Protein

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.56
MDL number:
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
300
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Immunogen

synthetic peptide corresponding amino acids 3-17 of GFP.

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:
  • in immunoblotting
  • in the histochemistry and immunohistochemistry
  • in the immunoprecipitation


Biochem/physiol Actions

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.
Specific for GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


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Classe de stockage

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Contenu apparenté


Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros
Liu Y, et al.
Development, 134(6), 1111-1122 (2007)
Green fluorescent protein: a perspective
Remington SJ
Protein Science, 20(9), 1509-1519 (2011)
Pathogenic mechanism of recurrent mutations of SCN8A in epileptic encephalopathy
Wagnon JL, et al.
Annals of Clinical and Translational Neurology, 3(2), 114-123 (2016)