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Merck

H0135

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

Synonyme(s) :

11β,17α,21-trihydroxypregn-4-ène-3,20-dione, 4-pregnène-11β,17α,21-triol-3,20-dione, Composé F de Kendall, Substance M de Reichstein

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About This Item

Formule empirique (notation de Hill) :
C21H30O5
Numéro CAS:
50-23-7
Poids moléculaire :
362.46
UNSPSC Code:
12352209
NACRES:
NA.77
PubChem Substance ID:
24895394
EC Number:
200-020-1
Beilstein/REAXYS Number:
1354819
MDL number:
MFCD00011654
biological source:
synthetic (organic)
form:
powder
assay:
≥80% (HPLC)

Nom du produit

Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

SMILES string

[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO

biological source

synthetic (organic)

sterility

γ-irradiated

product line

BioXtra

assay

≥80% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

mp

211-214 °C (lit.)

solubility

ethanol: 1 mg/mL, clear

shipped in

ambient

storage temp.

room temp

Quality Level

200

Gene Information

human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)

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Catégories apparentées

Biochem/physiol Actions

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
Principal glucocorticoïde secrété par la glande cortico-surrénale.

Application

Hydrocortisone is used for the following applications:
  • Used in myogenic induction and identification
  • Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
  • Used in adipogenic differentiation (one of the components in adipogenic medium)
  • Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
  • The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
  • Used in differentiation assays of melanoma spheroid cells

General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Preparation Note

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Repr. 1A - STOT RE 2

target_organs

Adrenal gland,Bone marrow

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificats d'analyse (COA)

Lot/Batch Number
0000469978
0000469978
0000401913
0000401913
0000382678

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Dong Fang et al.
Cancer research, 65(20), 9328-9337 (2005-10-19)
Recent studies suggest that cancer can arise from a cancer stem cell (CSC), a tumor-initiating cell that has properties similar to those of stem cells. CSCs have been identified in several malignancies, including those of blood, brain, and breast. Here
Q L Hao et al.
Blood, 86(10), 3745-3753 (1995-11-15)
We present cell cycling and functional evidence that the CD34+CD38- immunophenotype can be used to define a rare and primitive subpopulation of progenitor cells in umbilical cord blood. CD34+CD38- cells comprise 0.05% +/- 0.08% of the mononuclear cells present in
K L van Golen et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 5(9), 2511-2519 (1999-09-28)
Inflammatory breast cancer is a rapidly growing, distinct form of locally advanced breast cancer that carries a guarded prognosis. To identify the genes that contribute to this aggressive phenotype, we compared under- and overexpressed sequences in an inflammatory breast tumor
Andrej Jedinak et al.
International journal of oncology, 33(6), 1307-1313 (2008-11-21)
In spite of the global consumption of mushrooms, only two epidemiological studies demonstrated an inverse correlation between mushroom intake and the risk of cancer. Therefore, in the present study we evaluated whether extracts from edible mushrooms Agaricus bisporus (portabella), Flammulina
Oscar K Lee et al.
Blood, 103(5), 1669-1675 (2003-10-25)
It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different
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