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M7279

N,N′-Methylenebisacrylamide

Name: <I>N</I>,<I>N</I>′-Methylenebisacrylamide
Brand: SIGMA

powder, Molecular Biology, suitable for electrophoresis, ≥99.5%

Synonyme(s) :

BIS

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A propos de cet article

Formule linéaire :

(CH₂=CHCONH)₂CH₂

Numéro CAS:

110-26-9

Poids moléculaire :

154.17

UNSPSC Code:

41105319

NACRES:

NB.22

PubChem Substance ID:

24897151

EC Number:

203-750-9

Beilstein/REAXYS Number:

1706297

MDL number:

MFCD00008625

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Propriétés

grade

Molecular Biology

Quality Segment

200

assay

≥99.5%

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.05% Free carboxylic acid (T), ≤50 mg/kg Total sulfur (as SO₄) (ICP)

conductivity

≤10 μmho (2% solution)

anion traces

chloride (Cl^-): ≤50 mg/kg

cation traces

Ca: ≤10 ppm, Cd: ≤5 ppm, Co: ≤5 ppm, Cr: ≤5 ppm, Cu: ≤5 ppm, Fe: ≤5 ppm, K: ≤50 ppm, Mg: ≤5 ppm, Mn: ≤5 ppm, Na: ≤50 ppm, Ni: ≤5 ppm, Pb: ≤5 ppm, Zn: ≤5 ppm

absorption

≤0.2 at 290 at 1%

foreign activity

DNase and RNase, none detected

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

Description

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Application

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

N,N′-Methylenebisacrylamide has been used:

as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
as a crosslinker to prepare tough hydrogel network
in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

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pictograms

GHS06,GHS08

signalword

Danger

hcodes

H301,H312,H340,H350,H361fd,H372

pcodes

P202 - P260 - P264 - P280 - P301 + P310 - P302 + P352 + P312

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c