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Merck

08714

Methyl Red solution

suitable for microbiology

Synonym(s):

Methyl red indicator solution

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About This Item

Empirical Formula (Hill Notation):
C15H15N3O2
CAS Number:
493-52-7
Molecular Weight:
269.30
UNSPSC Code:
41171621
NACRES:
NA.85
PubChem Substance ID:
329748627
MDL number:
MFCD00002425
Beilstein/REAXYS Number:
1843037

Key Documents

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Product Name

Methyl Red solution, suitable for microbiology

InChI

1S/C15H15N3O2/c1-18(2)12-9-7-11(8-10-12)16-17-14-6-4-3-5-13(14)15(19)20/h3-10H,1-2H3,(H,19,20)/b17-16+

InChI key

CEQFOVLGLXCDCX-WUKNDPDISA-N

SMILES string

CN(C)c1ccc(cc1)\N=N\c2ccccc2C(O)=O

agency

according to GB 4789.30-2016
according to ISO 22964:2017

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

dist. water, 200 mL
ethanol 95%, 300 mL
methyl red, 0.1 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

Enterococcus spp.
Escherichia coli
Klebsiella spp.
Proteus spp.
Pseudomonas spp.
Staphylococcus spp.
bacteria

Quality Level

200

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Application

Methyl red solution is a pH indicator dye recommended for detection of bacteria producing acid from glucose fermentation such as Enterobacteriaceae in various samples.

General description

Methyl red solution is an azo dye which turns to red when pH is below 4.4 (yellow pH < 6.2, orange pH 4.4-6.2). Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red. This test is used to differentiate enteric bacteria.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

hcodes

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

69.8 °F

flash_point_c

21 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM8672
BCCL8848
BCCL0746
BCCK1481
BCCJ3868

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Chan-Ju Wang et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 66(Pt 1), 2-7 (2010-01-09)
Azoreductase 1 from Pseudomonas aeruginosa strain PAO1 (paAzoR1) catalyses the activation of the prodrug balsalazide and reduces the azo dye methyl red using reduced nicotinamide adenine dinucleotide cofactor as an electron donor. To investigate the mechanism of the enzyme, a
Oana Alexandru et al.
Journal of neuro-oncology, 102(1), 9-18 (2010-07-17)
A major focus of brain cancer research today is to translate understanding of glioma biology into advances in treatment, by exploring the potential of target therapy. Here we investigated the ability of three compounds belonging to the chemical class of
Sofia Babanova et al.
Journal of bioscience and bioengineering, 112(4), 379-387 (2011-07-26)
Soluble artificial mediators are often applied to enhance the electron transfer from living cells to an anode in microbial fuel cells. Recently, we have demonstrated that the Candida melibiosica 2491 yeast strain possesses electrogenic properties and can be used as
S S Gomare et al.
Journal of applied microbiology, 106(3), 993-1004 (2009-02-04)
To evaluate the potential of Brevibacillus laterosporus MTCC 2298 for the decolourization of different textile azo dyes including methyl red, mechanism of biotransformation and the toxicity of products. Brevibacillus laterosporus showed decolourization of thirteen different azo dyes including methyl red.
X D Zhang et al.
Journal of hazardous materials, 170(2-3), 883-887 (2009-06-09)
An aqueous C.I. Acid Red 2 solution was decolorized by electrolysis using iron as anode. The decolorization mechanism was investigated through experimental observations on the electrochemical behavior of C.I. Acid Red 2 on Pt rotating disk electrode, UV-visible spectra of
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