79378
Phosphate buffered saline
solution, pH 7.1-7.3, BioUltra
Synonym(s):
PBS
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About This Item
Product Name
Phosphate buffered saline, BioUltra, solution
product line
BioUltra
Quality Level
form
solution
impurities
insoluble matter, passes filter test
pH
7.1-7.3
cation traces
Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
General description
Phosphate buffered saline is a non-toxic biological buffer generally containing sodium phosphate, sodium chloride, and potassium phosphate. It prevents cells from osmosis-mediated rupture or dehydration. PBS is widely used for washing cells, immunohistochemistry, and various other cellular techniques.
Application
Phosphate buffered saline has been used in sample preparation for quantifying the wound fluid proteins from pressure ulcers in patients with spinal cord injury (SCI). It has also been used for cell rinsing in various biological researches.
Physical form
150 mM sodium phosphate; 150 mM NaCl; pH 7.2±0.1 (25°C)
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signalword
Warning
hcodes
pcodes
Hazard Classifications
Eye Irrit. 2
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Faceshields, Gloves, Goggles
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Laura E Edsberg et al.
The journal of spinal cord medicine, 38(4), 456-467 (2014-06-27)
To determine whether the biochemistry of chronic pressure ulcers differs between patients with and without chronic spinal cord injury (SCI) through measurement and comparison of the concentration of wound fluid inflammatory mediators, growth factors, cytokines, acute phase proteins, and proteases.
Marco L Leung et al.
Nature protocols, 11(2), 214-235 (2016-01-08)
Single-cell DNA sequencing methods are challenged by poor physical coverage, high technical error rates and low throughput. To address these issues, we developed a single-cell DNA sequencing protocol that combines flow-sorting of single nuclei, time-limited multiple-displacement amplification (MDA), low-input library
N C Martin et al.
Science & justice : journal of the Forensic Science Society, 46(3), 179-184 (2007-03-29)
In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the