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Merck

RDD035

HEPES sodium salt

≥99.5% (titration), free-flowing, Redi-Dri™

Synonym(s):

HEPES Sodium, HEPES hemisodium salt, Sodium HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
UNSPSC Code:
12161700
NACRES:
NA.25
MDL number:
MFCD00036463

Key Documents

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Product Name

HEPES sodium salt, ≥99.5% (titration), free-flowing, Redi-Dri™

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

product line

Redi-Dri™

assay

≥99.5% (titration)

quality

free-flowing

useful pH range

6.8-8.2

pKa (25 °C)

7.5

application(s)

diagnostic assay manufacturing

Quality Level

100

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Application

HEPES sodium salt can be used:
  • as a protein precipitation buffer
  • in cell culture media
  • as a binding buffer

Features and Benefits

  • Redi-Dri™ eliminates clumping for hygroscopic chemicals
  • powder remains free-flowing and easy to use
  • Safer to handle and weigh out for standard use in lab
  • Eliminates clumps, maintains product moisture, quality, and characteristics

General description

HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO2. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.

Other Notes

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Physical form

Lyophilized, spray dried, or crystallized material.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000506533
0000314581
SLCF3906

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NkBgl, a β-glucosidase from Neotermes koshunensis, is a β-retaining glycosyl hydrolase family 1 enzyme that cleaves β-glucosidic linkages in disaccharide or glucose-substituted molecules. β-Glucosidases have been widely used in several applications. For example, mutagenesis of the attacking nucleophile in β-glucosidase
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