A9205
Bovine Serum Albumin solution
30% in saline, fatty acid free, aseptically filled
Synonym(s):
Albumin solution from bovine serum, BSA solution
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About This Item
Biological source:
bovine
Assay:
28-32% protein basis (biuret)
Form:
solution
Technique(s):
ELISA: suitable, electrophoresis: suitable, western blot: suitable
Impurities:
≤0.01% free fatty acid
Concentration:
28-32% protein (biuret), 30% in saline
biological source
bovine
Quality Level
sterility
aseptically filled
assay
28-32% protein basis (biuret)
form
solution
purified by
cold ethanol fractionation
does not contain
stabilizer or preservative
origin
USA origin
concentration
28-32% protein (biuret), 30% in saline
free fatty acid content
≤0.01%
technique(s)
ELISA: suitable, electrophoresis: suitable, western blot: suitable
impurities
≤0.01% free fatty acid
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.
Application
Bovine Serum Albumin solution has been used:
- as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
- as a component of hepatocyte recovery medium
- as a component of fusion and activation buffers for in vitro reconstructed sheep embryos
Biochem/physiol Actions
Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.
Preparation Note
Initial fractionation by cold alcohol precipitation
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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