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Merck

A9576

Bovine Serum Albumin solution

30% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
28-32% total protein basis (UV)
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
endotoxin, tested
Concentration:
in phosphate buffer (DPBS)
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biological source

bovine

Quality Level

sterility

sterile-filtered

product line

BioXtra

assay

28-32% total protein basis (UV)

form

liquid

origin

USA origin

concentration

in phosphate buffer (DPBS)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile on Raw material, none detected

shipped in

ambient

storage temp.

2-8°C

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

BSA 30% solution is a convenient form for direct supplementation of serum-free media. Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.

Preparation Note

Prepared from A9647 in DPBS (D5652).


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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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J A A Pires et al.
Journal of dairy science, 102(6), 5347-5360 (2019-03-25)
The objective was to assess effects of experimentally induced undernutrition on responses to an intramammary lipopolysaccharide (LPS) challenge in early-lactation cows. Starting at 24 ± 3 d in milk, multiparous Holstein cows either received a ration containing 48% straw for
Paul W Kriebel et al.
The Journal of cell biology, 217(8), 2891-2910 (2018-06-10)
Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicles of multivesicular
Yasuhiro Kawai et al.
PloS one, 13(5), e0196655-e0196655 (2018-05-26)
Serum albumin is the major intravascular antioxidant. Though oxidative stress plays an important role in the pathophysiology of Immunoglobulin A nephropathy (IgAN), the association between serum albumin and the progression of IgAN is not entirely understood. This retrospective cohort study