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G1544

Anti-GFP, N-terminal antibody produced in rabbit

Name: Anti-GFP, N-terminal antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Green Fluorescent Protein

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD05665217

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

300

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Properties

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Immunogen

synthetic peptide corresponding amino acids 3-17 of GFP.

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:

in immunoblotting
in the histochemistry and immunohistochemistry
in the immunoprecipitation

Biochem/physiol Actions

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

Specific for GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000425859

0000382649

0000350272

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