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Merck

L0144

Lucifer Yellow CH dipotassium salt

Intracellular staining dye, powder

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About This Item

Empirical Formula (Hill Notation):
C13H9K2N5O9S2
CAS Number:
71206-95-6
Molecular Weight:
521.57
NACRES:
NA.77
PubChem Substance ID:
24896249
UNSPSC Code:
12171500
MDL number:
MFCD00013445
Beilstein/REAXYS Number:
5224942

Key Documents

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Product Name

Lucifer Yellow CH dipotassium salt, powder

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

form

powder

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

Quality Level

100

Related Categories

General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells. It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology
  • to assess intercellular communication activity through gap junction by scrape-loading assay
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
  • in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT2653
0000438866
0000438866
SHBR8105
SHBR1397

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A co-culture model for determining the target specificity of the de novo generated retinal ganglion cells
Teotia P, et al.
Bio-protocol, 7(7) (2017)
Menachem Hanani
Journal of cellular and molecular medicine, 16(1), 22-31 (2011-07-12)
The fluorescent dye Lucifer yellow (LY) was introduced in 1978, and has been extremely useful in studying cell structure and communications. This dye has been used mostly for labelling cells by intracellular injection from microelectrodes. This review describes the numerous
Kun-Yi Hsin et al.
Nucleic acids research, 39(Database issue), D1042-D1048 (2010-11-06)
We present the relational database EDULISS (EDinburgh University Ligand Selection System), which stores structural, physicochemical and pharmacophoric properties of small molecules. The database comprises a collection of over 4 million commercially available compounds from 28 different suppliers. A user-friendly web-based
In vitro mesenchymal-epithelial transition in NIH3T3 fibroblasts results in onset of low-dose radiation hypersensitivity coupled with attenuated connexin-43 response
Mathur A, et al.
Biochimica et biophysica acta. General subjects, 1862(3), 414-426 (2018)
Shuaiwei Wang et al.
Mediators of inflammation, 2020, 8094347-8094347 (2020-12-10)
Connexin (Cx) family members form hemichannels (HCs) and gap junctions (GJs). Biological functions of Cx HCs have not been adequately characterized due to the inability to selectively target HCs or GJs. Recently, we developed a 6-mer peptide mimetic (P5) of
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