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Merck

L9006

Lectin from Ulex europaeus (gorse, furze)

FITC conjugate, lyophilized powder

Synonym(s):

Ulex europaeus agglutinin, UEA-I

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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conjugate

FITC conjugate

Quality Level

form

lyophilized powder

potency

<5 μg/mL agglutination activity

composition

Protein, ~10% Lowry

extent of labeling

2-5 mol FITC per mol protein

storage temp.

−20°C

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Physical form

Contains potassium phosphate buffer salts and NaCl

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Other Notes

Conjugates are prepared from affinity purified lectin.


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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