M2634
Malic Dehydrogenase from porcine heart
buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)
Synonym(s):
L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase
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About This Item
Specific activity:
600-1000 units/mg protein (biuret)
form
buffered aqueous glycerol solution
specific activity
600-1000 units/mg protein (biuret)
cation traces
NH4+: ≤10 μg/mg protein
foreign activity
Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Application
Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.
Biochem/physiol Actions
Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.
General description
Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.
Mitochondrial Isozyme
Other Notes
One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.
Physical form
Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Nicholas R Moody et al.
Bio-protocol, 11(24), e4264-e4264 (2022-01-29)
Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly important in the context of C4 photosynthesis, where it
S S Chen et al.
The Biochemical journal, 151(2), 297-303 (1975-11-01)
1. Pig heart mitochondrial malate dehydrogenase incubated with pyridoxal 5'-phosphate at pH 8.0 and 25 degrees C gradually loses activity. Such inactivation can be largely reversed by dialysis or by addition of L-lysine or L-cysteine, and can be made permanent
X A Zhan et al.
Poultry science, 86(4), 654-660 (2007-03-21)
The effect of early feed restriction on metabolic programming and compensatory growth was studied in broiler chickens. A total of 480 female 1-d-old broiler birds (Aconred) were randomly allocated to ad libitum and feed-restricted groups, each of which was replicated
Comparative molluscicidal action of extract of Ginko biloba sarcotesta, arecoline and niclosamide on snail hosts of Schistosoma japonicum
Chen, S., et al.
Pesticide Biochemistry and Physiology, 89, 237-241 (2007)
Tomoko Matsuda et al.
Journal of pharmacological sciences, 112(3), 320-326 (2010-02-23)
We have reported that the differentiation-inducing factors (DIFs) DIF-1 and DIF-3, morphogens secreted from Dictyostelium discoideum, inhibit proliferation of several cancer cells via suppression of the Wnt/beta-catenin signaling pathway. However, the target molecules of DIFs involved in the anti-proliferative effects
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