M4892
McCoy′s 5A Medium
Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
5A basal medium
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About This Item
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.
Application
McCoy′s 5A Medium has been used for culturing:
- human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
- human colorectal HCT116 cells to study miR-224 over-expression in cancer
- osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Biochem/physiol Actions
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Preparation Note
Formulated to contain 11.9 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
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Related Content
Instructions
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
Yan Zhang et al.
Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study