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Merck

SAB4200175

Anti-Atg101 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Atg13-interacting protein, Anti-C12orf44, Anti-autophagy-related protein 101, Anti-chromosome 12 open reading frame 44

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
WB
Species reactivity:
human
Citations:
12
Technique(s):
western blot: 0.25-0.5 μg/mL using whole extracts of HEK-293T cells over expressing human Atg101

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~30 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

western blot: 0.25-0.5 μg/mL using whole extracts of HEK-293T cells over expressing human Atg101

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... C12orf44(60673)

Application

Anti-Atg101 antibody has been used in
  • immunoblotting
  • immunofluorescence
  • immunopurification

Biochem/physiol Actions

Autophagy101 (Atg101) is a mammalian Autophagy13 (Atg13) binding protein essential for autophagy. Atg13 forms a stable complex with ULK1 (Serine/threonine-protein kinase ULK1) and FIP200 (FAK family kinase-interacting protein of 200 kDa). Atg101 associates with the ULK1-Atg13- FIP200 complex through direct interaction with Atg13. Mammalian target of rapamycin (mTOR) interacts with the ULK1-Atg13-FIP200 complex in a nutrient dependent manner, suggesting that mTOR regulates autophagy through this complex.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Autophagy101 (Atg101) is located on the human chromosome at 12q13.13.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
068M4847V
060M4803

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Mei Yang et al.
Science advances, 2(9), e1601167-e1601167 (2016-09-13)
The intronic GGGGCC hexanucleotide repeat expansion in chromosome 9 open reading frame 72 (C9ORF72) is a prevalent genetic abnormality identified in both frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Smith-Magenis syndrome chromosomal region candidate gene 8 (SMCR8) is a
Expression of a ULK1/2 binding-deficient ATG13 variant can partially restore autophagic activity in ATG13-deficient cells
Hieke N, et al.
Autophagy, 11(9), 1471-1483 (2015)
Systematic analysis of ATG13 domain requirements for autophagy induction
Wallot-Hieke N, et al.
Autophagy, 14(5), 743-763 (2018)
Molecular Machinery and Genetics of the Autophagy Pathway
Autophagy in health and disease, 16-16 (2012)
Atg101, a novel mammalian autophagy protein interacting with Atg13
Hosokawa N, et al.
Autophagy, 5(7), 973-979 (2009)
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