T3405
3,3′,5,5′-Tetramethylbenzidine dihydrochloride
chromogenic, tablet
Synonym(s):
4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
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About This Item
Empirical Formula (Hill Notation):
C16H20N2 · 2HCl
CAS Number:
Molecular Weight:
313.27
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
264-769-6
MDL number:
Product Name
3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet
Quality Level
form
tablet
mp
≥300 °C
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChI key
NYNRGZULARUZCC-UHFFFAOYSA-N
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.
3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
- anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
- anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
- in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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