T3411
Tetramethylammonium chloride solution
Molecular Biology
Synonym(s):
N,N,N-Trimethylmethanaminium chloride
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About This Item
CAS Number:
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12352107
MDL number:
grade
Molecular Biology
Quality Level
concentration
5 M
foreign activity
DNase, RNase, none detected
SMILES string
[Cl-].C[N+](C)(C)C
InChI
1S/C4H12N.ClH/c1-5(2,3)4;/h1-4H3;1H/q+1;/p-1
InChI key
OKIZCWYLBDKLSU-UHFFFAOYSA-M
General description
Tetramethylammonium binds AT-rich DNA polymers while concomitantly abolishing the preferential melting of AT versus GC base pairs. It is supplied as a 0.2 μm filtered solution in 18 megohm water.
Application
Tetramethylammonium chloride solution (TMAC) has been used:
- in the preparation of hybridization cocktail for array hybridization and scanning
- in next-generation sequencing (NGS), and genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) library preparation
- in the preparation of TMAC buffer and bead hybridization mixture for hybridization and detection
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Yanwen Guo et al.
Methods in molecular biology (Clifton, N.J.), 1176, 33-44 (2014-07-18)
As small noncoding RNAs, microRNAs (miRNAs) regulate diverse biological functions, including physiological and pathological processes. The expression and deregulation of miRNA levels contain rich information with diagnostic and prognostic relevance and can reflect pharmacological responses. The increasing interest in miRNA-related
Nikolay L Malinin et al.
Nature protocols, 16(12), 5592-5615 (2021-11-14)
Genome-wide unbiased identification of double-stranded breaks enabled by sequencing (GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of genome-editing nucleases in living cells. GUIDE-seq is based on the principle of efficient integration of an end-protected double-stranded oligodeoxynucleotide
W B Melchior et al.
Proceedings of the National Academy of Sciences of the United States of America, 70(2), 298-302 (1973-02-01)
Several small alkylammonium ions can eliminate, or even reverse, the usual dependence of the DNA transition temperature on base composition. For example, in 3 M tetramethylammonium chloride, or 2.4 M tetraethylammonium chloride, DNAs of different base compositions all melt at