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Merck

T9378

Trypsin inhibitor

crude powder

Synonym(s):

Trypsin Inhibitor from lima beans

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About This Item

CAS Number:
9035-81-8
UNSPSC Code:
12352202
NACRES:
NA.77
EC Number:
232-906-9
MDL number:
MFCD00082102

Key Documents

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Product Name

Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder

biological source

Phaseolus limensis (lima bean)

type

Type II-L

form

crude powder

mol wt

9 kDa

solubility

0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL

storage temp.

2-8°C

Quality Level

200

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Analysis Note

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Application

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:
  • in the inhibition of trypsin in human plasma
  • in the termination of proteolytic digestion in rabbit skeletal muscles
  • in the inhibition of salivary glands enzyme extract from Lygus lineolaris
  • in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Biochem/physiol Actions

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

General description

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Other Notes

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.
View more information on Trypsin Inhibitor.

Preparation Note

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000515851
0000515851
0000507134
0000507133
0000507133

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A Rapid and Reliable Method for Total Protein Extraction from Succulent Plants for Proteomic Analysis
Lledias F, et al.
The Protein Journal, 36(4), 308-321 (2017)
Are cardiovascular and sympathoadrenal effects of human ?new pressor protein? preparations attributable to human coagulation beta-FXIIa?
Papageorgiou PC, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 286(3), H837-H846 (2004)
Shark skeletal muscle tropomyosin is a phosphoprotein
Hayley M, et al.
Journal of Muscle Research and Cell Motility, 29(2-5), 101-107 (2008)
Malik Shoaib Ahmad et al.
Proteins, 91(1), 22-31 (2022-08-05)
Bovine pancreatic trypsin was crystallized, in-complex with Lima bean trypsin inhibitor (LBTI) (Phaseolus lunatus L.), in the form of a ternary complex. LBTI is a Bowman-Birk-type bifunctional serine protease inhibitor, which has two independent inhibitory loops. Both of the loops
Partial characterization of trypsin-like protease and molecular cloning of a trypsin-like precursor cDNA in salivary glands of Lygus lineolaris
Zeng F, et al.
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 131(3), 453-463 (2002)
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